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C2C12 Cells (GFP11 Clone)
|Recommended Seeding Density||Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.|
|Markers||MHC and myogenin|
|Applications||For Research Use Only|
|Description||C2C12 Cells (GFP11 Clone) was established through transfection of retrovirus vector to carry the eleventh β-strand of GFP fused to the c-terminus of FLAG-14-3-3. The cells express GFP once myofusion occurs. It is a valuable tool along with the C2C12 Cells (GFP1-10 Clone) (Cat. No. T8003) as they function as a split GFP-based assay to evaluate compounds that promote myofusion and myogensis, and to study the diseases related to muscle loss such as muscle atrophy.|
|Propagation||The base medium for this cell line is Prigrow III medium available from abm (TM003). To make the completed growth medium, add the following components to the base medium: fetal bovine serum (TM999) to final concentration of 10% and 1% Penicillin/Streptomycin (G255). Atmosphere: air: 95%, CO₂: 5%; Temperature: 37.0°C.|
|Reference||Kodaka, M., Yang, Z., Nakagawa, K., Maruyama, J., Xu, X., Sarkar, A., . . . Hata, Y. (2015). A new cell-based assay to evaluate myogenesis in mouse myoblast C2C12 cells. Experimental Cell Research, 336(2), 171-181. doi:10.1016/j.yexcr.2015.06.015|
|Disclaimer||1. The CoA for this product (provided upon request) verifies the cell-type specific gene expression via RT-PCR only. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final.
2. We strongly recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping).
3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information.
4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.